01:119:115 Lecture Notes - Lecture 16: Lactose Permease, Beta-Galactosidase, Lac Operon
24 Dec 2015
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Document Summary
Occurs mainly at the transcriptional level (dna rna) Lac operon: introduction, terms enzyme response to, operon functions plus them transcription, example: lac operon, promoter nucleotide sequence; rna polymerase binds . Rna polymerase only binds, it does not transcribe: operator (short stretch of dna) nucleotide sequence; site, not transcribed, 3 structural genes- protein coding sequences. Hydrolyze lactose into glucose and galactose: lactose permease transmembrane transport carrier, beta- galactosidase (b-gal, galactoside transacetylase enzyme, genes lie next to each other on the chromosome and single promoter. All of the info from the three genes are going to be transcribed into a single rna strand: grow e. coli in a culture they share a (all or nothing) October 23, 2014 enzymes to be low begins enzymes to use the lactose. On medium with no lactose levels of all three are going. Binding site on lac operator for repressor protein. Rna polymerase has to bind to the promoter but can"t the structural genes because repressor.
