01:119:116 Lecture Notes - Lecture 2: Gram Staining, Crystal Violet, Atp Synthase
Document Summary
Hypothesis of first origin of life: prebiotic soup and iron-sulfur. 2 domains: bacteria and archaea: internal organization. Cell wall functions: protection, maintain cell shape, prevents bursting in hypotonic solution. 2 step process stain wash to id bacteria: It is more effective against gram + bacteria: motility. Does not hold violet, outer membrane of lipopolysaccharides(lps) H+ and the etc-similar to the etc in respiration that drives atp synthase. A proton gradient drives the movement of a protein. Bacterial/archean flagellum-differ only by proteins used (figure 3) Factors promoting genetic diversity in prokaryotes: rapid reproduction and mutation. Two factors give rise to high levels of genetic diversity. Diversity: mutations in the dna; very slow and gradual. Mutation in a gene is 1 in 10 million chance. In us: meiosis and crossing over figure 3 figure 4. Mutations are not enough to explain all of the diversity that we see.