14:125:201 Lecture Notes - Lecture 7: Beta Sheet, Quaternary, Alpha Helix
8 Oct 2016
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Document Summary
Number of chromosomes in human cells = 46. Number of chromosomes in human ovary / sperm cells = 23. Number of chromosomes in onion cells = 16. Number of chromosomes in fruit fly cells = 8. Number of chromosomes in e coli cells = 1. Site-specific mutagenesis: alteration of a sequence at a single site for a desired result. Adeno-associated virus (aav) delivery system: use of an adeno virus carrying a strand of dna to be delivered to a cell as a treatment. Pregnancy test: uses antibodies to detect proteins and evaluate pregnancy. Number of cells in body: 20 million. Sanger method: more efficient method to sequence dna. Blast: database with dna sequences of known / researched organisms. Cycle sequencing: use of a thermal cycler to perform repeated synthesis reactions. Shotgun cloning: use of restriction enzymes to cut a desired strand of dna and to sequence them simultaneously. Northern blot: evaluates characteristics of rna. Southern blot: evaluates characteristics of dna.
