01:694:215 Lecture Notes - Lecture 1: Signal Transduction, Phosphodiester Bond, Lac Operon
26 Mar 2018
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Document Summary
Cell types have to express different types of genes different set of genes turned on or off differentially regulated. Some genes are turned on always- constitutive transcription. Genes are not regulated in the proper fashion. Dna transcription rna translation protein. Transcription mrna processed/degraded and transported into cytoplasm. Most regulation happens in transcription (atp required in subsequent steps, so why waste the energy?) Rna polymerase binds to promoter at dna and starts to unwind the. Dna and begins copying the strand complementary sequence. Separates the dna and creates a transcription bubble. Hits the terminator region and unbinds from dna. Rna will be 3 5 (requires okazaki fragments) Matches the sequence of the rna made from the template strand. Polymerase structure- cleft region is the site of action. Specificity- ability to discriminate between different things (bind to one. Affinity- strength of interaction between different things (bind one dna site stronger vs another) General transcription factors (gtfs)- specificity and affinity for.
