THEA 101 Lecture Notes - Lecture 4: Pineal Gland, Bioassay, Pituitary Gland
Document Summary
Exocrine glands: secrete substances to ducts body cavities/surfaces. Ex. sweat, oil, salivary, mucous, digestive glands. Endocrine glands: ductless glands that secrete hormones into bloodstream. Es: consists of hypothalamus, hypophysis (pituitary gland), pineal gland, gonads, placenta and other various parts in human body. Endocrinology: study of endocrine glands and secretions; includes study of paracrines (chemical messengers that act locally on adjacent target cells with receptors, not carried into blood). Bioassay: indirectly measures how much hormone is in a glandular tissue/blood. Preparation of removed gland), observe to see if replacement therapy reverses effect of gland removal. Remove gland, observe gland removal effect, replacement therapy (admin. Hplc and spectrophotometric analyses: identify hormones and regulators in tissue/blood by using antibody against hormone and radioactive tracer to detect and measure the hormone. Radioimmunoassay: direct and accurate way to measure how much hormone is in. Elisa assays: used for measuring variety of hormones and hormone products. Gas chromatography and mass spec: measure mixed steroids in plasma/urine.
