CHEM 31X Lecture Notes - Lecture 1: Electrospray Ionization, Peptide

• School: Stanford University
• Department: Chemistry
• Course: CHEM 31X
• Professor: Coxand Zare

Document Summary

Small molecules can"t have too much of a positive charge: can"t be held together; coloumb"s attraction keeps the molecule together, long molecules like polypeptide can have diverse charge. Plot of intensity vs mass/charge: intensity = number of ions per second, charge = 1, if leak air, you will see peak in nitrogen (n, and (much lower) peak in oxygen (o+ 2: if you put in cl2 then you will get three peaks (two main isotopes: 35 and 37) so peaks at 70, 72, and 74.