BIO 204 Lecture Notes - Lecture 13: Amplicon, Agarose, Podcast
12 May 2019
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Place tip of pipette over well and support with tips of your fingers when transferring. Dna is negatively charged so it will run against positive electrode. Be sure copper tongs touch contact plates to conduct current. Food that test positive will produce a 200 base pair band in the gmo amplification reaction and a 455 base pair amplicon in the plant primer reaction. Non-gm food produce an amplicon with the plant-specific primers, but not with the. Allow the gel to solidify for 20 mins, remove tray and wrap it in plastic wrap: running the amplicons on a gel, while gel sets, get pcr samples and thaw at room temperature. For power supply and gold tank connectors: switch to the low setting on the power supply (140 v) i. Be sure to see stream of bubbles coming from electrodes in tank, run for ~
