BIO 362 Lecture Notes - Lecture 21: Transesterification, Circular Rna, Snrnp
Document Summary
Polyadenylation purpose in bacteria to target rna for degradation; eukaryotes use capping and polyadenylation for stability. Cap consists of 7-(cid:373)eth(cid:455)l, gua(cid:374)osi(cid:374)e with u(cid:374)i(cid:395)ue li(cid:374)kage (cid:894)5",5"- t(cid:396)iphosphate(cid:895); (cid:1006)"oh has methylation stabilizes rna; cap-binding protein that interacts and protects know how. Guanylytransferase recruite via cdt of rna adds cap structure cap binding complex protects. 5"5" 7-methyl g cap; experiment with capped mrna + ribosomes on a 5:20 sucrose gradient (sep small and large subunits- larger migrates farther down) cap plays role in ribsome association, because a lot of ribosomes migrates down. Splicing- removal of introns- intervening noncoding seq- rna poly associates with c-term domain. Introns vary in eukaryotes- many diff roles; most prokaryotes do not have introns. Hybridization experiment- fragment genome that hybridized in dna there are seg that do not match rna blue segments end up in mature rna, while an active mechanism in cell that removes grey loops- splicing.
