BSC 114 Lecture Notes - Lecture 18: Dna Replication, Fibroblast, Spindle Apparatus

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Outside the nucleus, the microtubule spindle (aka spindle apparatus) assembles between two centrosomes. The chromosomes are aligned at the metaphase plate, the equator of the spindle midway between the spindle poles. The kinetochore microtubules attach sister chromatids to opposite poles. The sister chromatids synchronously separate to form two daughter chromosomes, and each is pulled slowly toward the spindle pole it faces. The kinetochore microtubules get shorter and the spindle poles also move apart due to net growth of the overlap (nonkinetochore) microtubules- both processes contribute to chromosome separation. The two sets of daughter chromosomes arrive at the poles of the spindle and decondense. Cytokinesis, the division of the cytoplasm, begins in animal cells, fungi, and many protists with the formation of the contractile ring. The cytoplasm is divided in two by the contractile ring, which pinches the cytoplas in two to create two daughter cells, each with one nucleus. The interphase microtubule array is nucleated by the centrosome.

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