BIO 201 Lecture Notes - Lecture 8: Endoplasmic Reticulum, Secretion, Cell Membrane
3 Apr 2017
School
Department
Course
Professor
Document Summary
Not interconnected with er, but similar structure. Divided into cis-, medial, and trans- stacks. Pancreatic acinar cells- produce and secrete copious amounts of digestive enzymes. Proteins made in rer; exit rer and move to golgi; exit golgi and end up in vesicles. Pulse chase experiment: labeling of proteins, for subcellular location. The secretory pathway: production of proteins in the rer. Constitutive: on all the time, slow and steady". Regulated (a. k. a. exocytosis): vesicles stored at plasma membrane until cell receives a stimulus. Exocytosis: vesicle proteins dock vesicle at plasma membrane but prevent fusion, ca++ causes conformational change of protein complex, forcing vesicle onto membrane -> fusion. Electrical impulse -> ca++ enters through channel on plasma membrane. Internal or external signal -> ca++ enters through channel in smooth. Primary lysosome and endosome will fuse in the cytoplasm and fuse spontaneously because they are lipids. Phagosome fuses with lysosome activating h+ pumps (h+ pumed in)
