BIO 201 Lecture Notes - Lecture 32: Differential Centrifugation, Centrifugal Force, Centrifugation
3 May 2017
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Parental strand is used as template for creation of new daughter strand. Each daughter cell inheriting half old, half new dna. Double stranded parental dna is retained in its entirety through replication and inherited by one of daughter cells. Cell reads double stranded sequence to create an entirely new copy of dna that is in sequence identical. Dna replicated in little bits and glued back together again. Labeled dna during replication with different isotopes of nitrogen (different densities) Identify density of dna at each generation using density gradient centrifugation! Particles whose mass is above a certain size pellet at selected centrifugal force. Good for separation of structures with very different masses. Particles settle where particle density matches solute density. Changing nitrogen isotopes changes density of dna: feed e. coli 15n (heavy n) for several generations, purify dna from 15n-fed e. coli and from 14n-fed e. coli, place both 15n and 14n into one tube and centrifuge.
