BIO 201LLB Lecture Notes - Lecture 24: Differential Centrifugation, C4 Carbon Fixation, Leaf

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C4 plants: have a spatial separation (area) Cam plants: have temporal separation (time: all happens in mesophyll cell, grown in different areas specialization. Chemical energy is stored in carbohydrates: 5% One piece of closed circular double-stranded bacterial dna. Only newly synthesized dna will be marked. Meselson-stahl experiments: grow bacteria on heavy nitrogen (15 n) to metabolically label all dna, dna denser because of heavy nitrogen, then switch to regular light nitrogen (14 n). All newly replicated dna will be light but the old parental dna will be heavy: used to tell difference between new and old dna. Density centrifugation: use density gradient centrifugation to separate heavy and light dna, they actually used cesium density gradients instead of sucrose. Add a mix of light, heavy, and hybrid dna to a cesium density gradient. Top to bottom: less dense (14 n) hybrid denser (15 n)

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