MIC 301 Lecture Notes - Lecture 4: Bright-Field Microscopy, Wavelength, Microbiology

• School: University at Buffalo
• Department: Microbiology And Immunology
• Course: MIC 301
• Professor: Dombroski Darlene

Document Summary

Visible light part of the spectrum of electromagnetic radiation. White light is composed of many colors (wavelengths), has an average wavelength of 550nm. Electrons moving electrons act as waves, with wavelength dependent on the voltage of the electron beam. There is a direct correlation between the smaller the wavelength produces the greatest enhancement of microscopy. I(cid:374)(cid:272)rease i(cid:374) size of a(cid:374) o(cid:271)je(cid:272)t (cid:449)hi(cid:272)h is i(cid:374)di(cid:272)ated (cid:271)(cid:455) a (cid:374)u(cid:373)(cid:271)er a(cid:374)d (cid:862)(cid:454)(cid:863) (cid:449)hi(cid:272)h (cid:373)ea(cid:374)s (cid:862)ti(cid:373)es(cid:863). Magnification is produced when a beam of radiation refracts (bends) when passed through a lens. Resolving power is the ability to measure the separation of images that are close together. The wavelength of visible light (400 _____> 700nm) limits the resolution. Contrast refers to differences in coloration or intensity of coloration between two objects or it"s (cid:271)a(cid:272)kgrou(cid:374)d. co(cid:374)trast of (cid:373)i(cid:272)roorga(cid:374)is(cid:373)s is a(cid:272)hie(cid:448)ed (cid:271)(cid:455) stai(cid:374)i(cid:374)g. Two lens systems: ocular/eyepiece lenses, objective lenses (primary lenses) Ocular lens re-magnifies the image formed by the objective lens.