MIC 401LEC Lecture Notes - Lecture 17: Obligate Anaerobe, Oxidative Phosphorylation, Cell Membrane

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Bacterial growth: binary fission, most grow in artificial culture. Elements needed: n2, h2, co2, c, po4, fe, s, k+, ca2+, mg2+, energy source. Prototroph: can derive all nutritional requirements for growth from micronutrients. Fastidious: microorganisms that have complex and special nutrients requirements (might need vitamins or amino acids) Oxygen: obligate aerobe: absolute requirement for o2, facultative aerobe/anaerobe: grow either aerobically or anaerobically, obligate anaerobe: ideal growth in absence of o2. Phases of bacterial growth: lag phase: initial, beginning of growth, logarithmic or exponential phase: increase in numbers, stationary phase: no increase in # of bacteria, death: decline in bacterial numbers. Energy > synthesize organic compounds needed by the cell. Heterotrophic: pathogen bacteria, get energy from organic compounds. Autotrophic: no sunlight, no organic compounds, use inorganic compounds. Bacteria may generate energy by: aerobic respiration: final electron acceptor (oxygen, glycolysis (glucose is primary substrate, pyruvate as primary end product) Investment phase: 2atp is used to make adp.

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