BIOE 120 Lecture Notes - Lecture 2: Microscopy, Plant, Organelle
21 Dec 2016
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Document Summary
Light microscope- 200nm minimum resolvable by light microscope (can start making out organelles. Brightness of an image is proportional to the amount of light (# of photons) that penetrate the specimen. Resolution- smallest distance between two points on a specimen that can be distinguished as two separate entities. Light microscopy: light source is visible light (white light, minimum resolvable distance is about 200nm, resolution- cell shape and nucleus, staining with colored dyes and fluorescent microscopy. Electron microscope: using electrons (e-) to illuminate the specimen, wavelength similar to an x-ray (0. 01-10nm, minimum resolvable distance is about 2nm, resolution= organelles, large molecules (ribosomes), and structure. Cells and cell structure: both, prokaryotes. Bacteria and archaea (live in extreme environments. Bacteria has a cell wall (not unique to prokaryotes) Cytosol (gelatinous fluid between the nucleus and the plasma membrane. Mitochondria: cytoplasm, everything between the nucleus and the plasma membrane (including the organelles, cytosol. Gelatinous fluid in cytoplasm, not including the organelles: nucleus.
