BSCI 223 Lecture Notes - Lecture 14: Multiple Drug Resistance, Antimicrobial Resistance, Pilus
25 Mar 2016
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Document Summary
Introducion of naked dna into a recipient bacteria either naturally or by inducion in the laboratory. Induced competence: requires high salt or electroporaion in the lab (e coli, can put cells in growth media or bufer and sill transform dna. Requires direct contact between two bacterial cells via a pilus. Dna in the donor cell is transferred into the recipient cell. Requires a speciic bacteriophage (virus) to transfer dna to a recipient bacteria during infecion. Conjugaive plasmid: contains all of the genes needed to transfer itself (conjugate) from current cell (donor) to new cell (recipient). These are usually low-copy number similar to chromosome. Gender of bacteria: f+ (male) carry f plasmid, make sex pilus, f- (female)- no f plasmid, no sex pilus. F (ferility): direct contact of an f+ donor male with an f- recipient female via the sex pilus results in transfer or conjugaion of the f plasmid.
