BSCI 330 Lecture Notes - Lecture 10: Eukaryotic Initiation Factor, Nonsense Mutation, Start Codon

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What does it take to make protein: transcription, translation, transcription, fixed set of dna molecules working rna copies, transcription components: Rna polymerase recruiting factors: rna the differences, ribose instead of deoxyribose. Know: mrnas, rrnas, trnas, snrnas, mirnas, sirnas: mrna: template for translation, rrna: structural component of ribosome, trna: translates mrna nucleotide sequences into amino acid sequence, transcription: rna polymerase. Rna polymerase: multi-subunit enzyme responsible for carrying out transcription. Catalyzes nucleotide polymerization within its active site. Energy stored in the phosphoanhydride bonds of the incoming ribonucleoside triphosphates powers polymerization. Holoenzyme = rna polymerase + sigma factor. Elongation = sigma factor + rna polymerase begin to read the nucleotide strand until it gets to a termination sequence. Termination in bacteria: there is a region that is rich in g-c"s in the mrna transcript, followed by a series of a"s in the dna template series of u"s in the mrna transcript hairpin loop.

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