CHM 205 Lecture Notes - Lecture 3: Boiling Point, High-Performance Liquid Chromatography, Silica Gel

• School: University of Miami
• Department: Chemistry
• Course: CHM 205
• Professor: Eve T M

Document Summary

Column chromatography separation technique based on solid-liquid phase partitioning. Used for obtaining compounds from natural sources of purifying products from rxn mixtures. Stationary phase any material that doesn"t dissolve in the associated liquid phase. Thin layer of adsorbent attached to a solid support. Column is filled with a larger amt. of adsorbent and mixture is loaded on top of it. An eluent is allowed to percolate through the column by gravity. As the eluent is moving down the column, it carries the soluble compounds. Compounds with stronger interaction with solvent move more slowly. Compounds will separate in bands and each band will come out of the column. You will separate a mixture of ferrocene and acetylferrocene. Selecting a column (for smalls samples with good separation you can use a pasteur pipet) Weight of adsorbent 20 times the weight of the crude sample. Use a cotton plug in the bottom of the pipet to keep adsorbent in column.