BIOL 1543 Lecture Notes - Lecture 18: Recombinant Dna, Dna Ligase, Plasmid
25 Aug 2016
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Gene cloning: biotechnology the manipulation of organisms or their components to make useful products. Used microbes to make wine and cheese. Selectively bred stock, dogs and other animals: dna technology set of modern techniques used to study and manipulate genetic material, genetic engineering inserting genes from one organism into another. Genetic engineering involves manipulating genes for practical purposes. Gene cloning leads to the production of multiple, identical copies of a gene-carrying piece of dna. Recombinant cells and organisms can mass-produce gene products. Recombinant cells and organisms constructed by dna technologies are used to manufacture many useful products, chiefly proteins: bacteria are often the best organisms for manufacturing a protein product because bacteria: Have plasmids and phages available for use as gene- cloning vectors. Can be engineered to produce large amounts of a particular protein. Often secrete the proteins directly into their growth medium. Mammalian cells must be used to produce proteins with chains of sugars (glycoproteins: examples:
