BIOL 3077 Lecture Notes - Lecture 6: Polyacrylamide Gel Electrophoresis, Capillary Electrophoresis, Multiplex Polymerase Chain Reaction
Document Summary
Distinguish between slab gels and capillary gels, conventional electrophoresis gels separates molecules as they migrate through a slab gel matrix, problem with slab / polyacrylamide gel is that their big and fragile, and cumbersome. Not everything on a big slab was perfectly straight: capillary electrophoresis separates molecules as they travel along the inside of a small capillary tube that is filled with a conductive, liquid buffer, rather than a gel. Simple mendelian inheritance and interpretation: even more simple than single-locus rflp. Fluorescent tag will give off light in visible spectrum: can be used on a slab gel, capillary gel electrophoresis, capillary tube filled with polyacrylamide gel, equivalent to 1 lane in slab gel. This one lane is contained in a capillary tube: as a fragment comes by, a detector shines light which excite flouroscent molecule that is then detected by a camera, makes a. Is 4 the practical limit of the number of str loci in a single multiplex.
