BI 328 Lecture Notes - Lecture 3: Immunohistochemistry, Acrosome, Ethylenediaminetetraacetic Acid

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Molecule/structure must be present at the right time/place. Part of the sperm that initially adheres to the egg. Put out from the sperm by polymeration of actin molecules. Thought to be part of the acrosome. Put an antibody against bindin with the cell. If the sperm had bindin anywhere on it, the antibody would bind. Protein dab would become blue-black precipitate when antibody bound. Bindin found to be part of acrosome. More inhibitors better at preventing sperm from binding to egg. Put bindin particles in solution with eggs. Juno binds izumi protein at the cell membrane. Inhibiting either izumi or juno inhibits fertilization. Juno needed for sperm egg binding in mammals. Embryonic kidney cells expressing juno, and embryonic kidney cells expressing izumi. When put together, they adhere to each other at izumi-juno boundary. Calcium ions spread through egg at fertilization, not before. Inhibit with edta, there is no egg activation.

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