BIO 112 Lecture Notes - Lecture 15: Methionine, Start Codon, Prokaryotic Small Ribosomal Subunit
1 Oct 2015
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When the poly a tail degrades, the mrna is destroyed. Removal of introns: alternate slicing allows for different versions of proteins from a single gene. Figuring out the genetic code: used and grinded up e. coli, got rid of the preexisting mrna and pulled out the ribosomes, added rna, new protein will be made. Added radioactive amino acids (to be able to detect which amino acids end up in the protein). Exoribonuclease (not really a polymerase): enzyme"s normal function is to break down. You can drive the reaction backwards for this enzyme to reverse reaction. However, the problem is that it doesn"t have a template and you can make a specific sequence. 3 stop codons tell to stop making the proteins and do not code for a protein. However, these experiments did not have a start codons so the experiments should not have worked. Prokaryotes: have simultaneous transcription and translation because they do not have a nuclear membrane.
