BIO SCI 98 Lecture Notes - Lecture 12: Citric Acid Cycle, Atp Synthase, Succinic Acid

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4 Apr 2016
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Chemiosmotic theory: e- passed through chain of carriers, protons pumped against concentration gradient proton motive force drives synthesis of atp. Intermembrane space (p + side) vs. mitochondrial matrix (n side: electron transport chain: e- flow from low to high reduction potential (releases energy thru e-, energy used to move protons against concentration gradient a. i. Ubiquione (q): 1 or 2e-; lipid-soluble, can move within the membrane a. i. 1. a. i. 2. a. i. 3. Nadh e- from complex i (10 h+ pumped into p side) Fadh2 e- from complex ii (6 h+ pumped into p side) glycerol 3-phosphate dehydrogenase (nadh shuttling) a. i. 3. a. cytosolic and mitochondrial enzymes 1. 5 atp a. i. 4. Malate aspartate shuttle: same as gluconeogenesis a. i. 4. a. a. i. 4. b. Oaa cannot cross the membrane malate oaa collects electrons to reform nadh 2. 5atp a. i. 5. Qh2 delivers the second e- to another q d. ii. Net: 1qh2 + 2 cyt c + 2h+ q + 2 cyt c + 4h+ (two protons: complex iv: cytochrome c to o2 e. i.

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