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Lecture 11

BIO SCI 99 Lecture Notes - Lecture 11: Rna Interference, Aconitase, Globin


Department
Biological Sciences
Course Code
BIO SCI 99
Professor
Olga Razorenova
Lecture
11

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Bio 99 Lecture #5 (part 2)
This lecture is based on chapter 22 pages 760-779 (post-transcription
control)
I. Development in multicellular eukaryotes
a. A adult hua has  tillio ells
b. All of these come a single fertilized egg cell
c. Nearly all have the same DNA, yet there are many organs and cell
types within organs that carry out specialized functions
d. Gene regulation allows different cell types to develop
II. Post-transcriptional control
a. tasiptioal otol: the deisio to iitiate tasiptio of
a gene
b. Regulation of expression at the level of mRNA synthesis
c. I additio to tasiptioal otol, a post-transcriptional
ehaiss egulate gee epessio i eukaotes
d. Some of these mechanisms include:
i. mRNA storage
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ii. alternative splicing
iii. multiple mRNA cleavage/ polyA sites
iv. regulation of ribosome initiation
v. siRNA/RNAi
III. tools for studying post-transcriptional control
a. atioi D ihiits TRAN“CRIPTION
i. it binds DNA at the transcription initiation complex and
prevents RNA polymerase elongation in eukaryotes
b. loheiide ihiits TRAN“LATION
i. Cycloheximide blocks the activity of eukaryotic ribosome
and therefore protein synthesis
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c.
IV. Post-transcriptional control
a. Yeast starved from sugar turning OFF of protein synthesis
b. Is this transcriptional or translational control?
c. otol: potei sthesis estoed ith suga additio
d. atioi D: potei sthesis estoed ith suga additio
e. No effect of inhibiting transcription or resuming protein
synthesis, therefore mRNAs must not be degraded during arrest
f. What ould loheiide do i this epeiet?
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