BIO SCI 99 Lecture Notes - Lecture 11: Rna Interference, Aconitase, Globin
17 May 2018
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Bio 99 Lecture #5 (part 2)
This lecture is based on chapter 22 pages 760-779 (post-transcription
control)
I. Development in multicellular eukaryotes
a. A adult hua has tillio ells
b. All of these come a single fertilized egg cell
c. Nearly all have the same DNA, yet there are many organs and cell
types within organs that carry out specialized functions
d. Gene regulation allows different cell types to develop
II. Post-transcriptional control
a. tasiptioal otol: the deisio to iitiate tasiptio of
a gene
b. Regulation of expression at the level of mRNA synthesis
c. I additio to tasiptioal otol, a post-transcriptional
ehaiss egulate gee epessio i eukaotes
d. Some of these mechanisms include:
i. mRNA storage
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ii. alternative splicing
iii. multiple mRNA cleavage/ polyA sites
iv. regulation of ribosome initiation
v. siRNA/RNAi
III. tools for studying post-transcriptional control
a. atioi D ihiits TRAN“CRIPTION
i. it binds DNA at the transcription initiation complex and
prevents RNA polymerase elongation in eukaryotes
b. loheiide ihiits TRAN“LATION
i. Cycloheximide blocks the activity of eukaryotic ribosome
and therefore protein synthesis
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c.
IV. Post-transcriptional control
a. Yeast starved from sugar → turning OFF of protein synthesis
b. Is this transcriptional or translational control?
c. otol: potei sthesis estoed ith suga additio
d. atioi D: potei sthesis estoed ith suga additio
e. No effect of inhibiting transcription or resuming protein
synthesis, therefore mRNAs must not be degraded during arrest
f. What ould loheiide do i this epeiet?
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