BIO SCI M122 Lecture 47: Urea Hydrolysis
4 Dec 2016
School
Department
Course
Professor
Document Summary
Describe a positive result of the urease test. An enzyme that has a role as a virulent factor. Splits urea into carbon dioxide and ammonia. Helicobacter pylori use urease to neutralize stomach acids, so that it can invade the mucosal cells of the stomach and establish disease such as stomach ulcers. Useful in the identification of gram-negative rods of the genera proteus & morganella. Organisms that produce the enzyme urease will break down the urea from the urea slant thereby releasing ammonia which reacts with the ph indicator phenol red. Describe a negative result of the urease test. A beige / salmon color (color of the slant you started with) Contains ingredients that provide a visual change in the media to distinguish between organisms that possess certain biochemical traits vs. those that don"t. They hydrolysis urea to carbon dioxide and ammonia. The indicator in this test is phenol red. When the solution becomes basic the phenol red becomes bright pink.
