BIO SCI D103 Lecture Notes - Lecture 10: Intermediate Filament, Protein Targeting, Tight Junction
18 Mar 2014
School
Department
Course
Professor
Document Summary
Ca2+ i. ii. b. into nucleus i. ii. iii. iv: cadherins. Embryonic structure - mixed cells - require cadherins to interact with. Ca2+ reveals the binding domains on the cadherins. Test if a n-terminus of a protein is necessary for directing protein. Remove n-terminus a. b. b. a. b. a. b. b. b. Also engages in bi-directional signaling. a. (http://www. biology. northwestern. edu/igp/faculty/green/: mediates the connection between f-actin of neighboring cells i. Used for motility and folding of the epithelial layer b. c. Apical and basolateral surfaces have different protein composition. Prevents molecules from the apical membrane to move ii. Separates the membrane proteins from mixing: protein transport within the cell. Release from machinery and folding a. a. b. b. c. d. e. a. b. a. b: protein sorting in cytosol. Cytosolic ribosome is transiently attached to the rer i. Only one pool of ribosomes: sorting signal. Attached sorting signal to gfp and see where gfp is taken b.
