BIOL 104 Lecture Notes - Lecture 2: Transmission Electron Microscopy, Oil Body, Elodea
9 Mar 2020
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Identify the cell walls: observe circular structures that could be vacuoles, amyloplasts, and other organelles, add an iodine (i2/ki) solution to one side of the specimen, starch grains inside the amyloplasts should be stained blue/black with iodine. Identify the cell walls, and the small, orange or red, disc shaped organelles: these are chromoplasts and these plastids store the pigment carotenoids. Exercise 4: avocado, cover with sudan iv or sudan black b, the stain will reveal the oil bodies since lipids stain pink (iv) or orange (b, oil bodies are storage structures for fats, avocadoes store abundant fat. In very ripe avocado fruit, cell walls have been digested and may not be visible. Exercise 7: measure the size of cells and their organelles of egeria, name plastids and other organelles and their functions. Identify the structures/organelles common to both plant and animal cells: plasma membrane, cytoplasm, golgi bodies (complexes of dictyosomes), oil (lipid) bodies, mitochondria, nucleolus, nucleus, and endoplasmic reticulum.
