LIFESCI 3 Lecture Notes - Lecture 7: Noncoding Dna, Okazaki Fragments, Hayflick Limit
Document Summary
Mechanism of dna replication: replication fork, dna is splitting and new strand is generated within that. The existing 3" oh group at the very end of the chain attacks the - template/parental strand phosphate from an incoming dntp: dna polymerase facilitates this attack, 4. The pyrophosphate (2 phosphate groups) leaves: this is the energy involved in adding a nucleotide to a growing strand of dna, 5. Proteins involved in dna replication: sliding clamp, refer to dna polymerase iii/parts of dna polymerase/ sliding clamp in notes. Topoisomerase: function: prevents/removes dna supercoiling in front of replication fork, as the helicase unwinds the dna, knotting in the dna can form. Primase: function: rna polymerase; lays the primer, process, as the dna is unwound, the primase binds to single-stranded. Eukaryotes: genomes are linear & vastly larger there are multiple origins of replication in the dna, increases the processivity/speed of dna replication, q: in contrast to linear dna, circular dna replication typically:
