LIFESCI 3 Lecture Notes - Lecture 14: Aminoacyl-Trna, Open Reading Frame, Start Codon
Document Summary
Aug"s throughout the protein: internal aug binds trna molecule with regular met. Degenerate genetic code: some amino acids have multiple codons, ex. Leucine: uua, uug, cuu, cuc, cua, cug: note: be able to read genetic code table (slide 7) Purpose: called an adaptor molecule , helps communicate and translate between mrna and protein, reads the mrna in codons of 3 bases and translates it into an amino acid. Structure: has a region that can base pair with 3 nucleotides on an mrna codon, very complex structure, 2d: cloverleaf shape, 3d: l-shape, they"re processed even though they"re not translated, ex. non-canonical modifications (i. e. uncommon bases) on trna. Discovery of the genetic code: in vitro translation experiment. Helped map out 4 codons for amino acids: it wasn"t until later that better techniques were developed to make. Q: a particular triplet of bases in the coding strand of dna is 5"-tga-3".
