LIFESCI 3 Lecture Notes - Lecture 11: Gene Expression, Deoxyribose, Uracil
Document Summary
Only certain part of genome turned on, those required for functions of that cell --> gene expression. A lot of genes do not make protein. Reads from 3" to 5", synthesizes from 5" to 3" Only accepts rntp monomers to form complementary rna strand. Only one strand is used as template strand/promotor from one gene. Segment of dna containing info for single polypeptide chain or functional rna. Opposite direction of which template dna strand is transcribed. The promotor is always upstream of the gene. When given sequence of gene - nontemplate strand (same as rna strand) Usually g/c rich followed by a rich sequence (on template) U is newly synthesized, a is part of sequence. Rna chain released b/c a-u is less stable, disassociated. 35 and - 10, spacing between is important. Alpha i, alpha ii, beta, beta prime, omega -> core enzyme. Core rna pol enzyme + sigma 70 factor = rna pol holoenzyme.
