LIFESCI 3 Lecture Notes - Lecture 5: Southern Blot, Electrophoresis, Insitu
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Summary of mains forms of enzyme regulation: When the end product inhibits enzymes used in early stages of the reaction. When an effector molecule binds to a site on an enzyme that is not the active site. When an inhibitor prevents the substrate from binding to an enzyme at the active site. Alteratio(cid:374)s i(cid:374) the e(cid:374)zy(cid:373)e"s structure of a(cid:373)ino acids, which can render enzymes inactive. The rate of enzyme synthesis and the rate of enzyme breakdown can affect enzyme activity. Regulatory molecules typically either mask the active site of an enzyme (preventing substrate from attaching to it) or reveal the active site (unblock it) In this course, phosphorylation (attaching phosphate groups) is one of the most significant forms of enzyme modification. It is important to be aware of different techniques used in molecular biology, this information will cumulatively be covered on exams. Summary of some techniques covered (these will be discussed in more detail later):
