LIFESCI 3 Lecture Notes - Lecture 6: Antibody, Sodium Dodecyl Sulfate, Immunofluorescence
Document Summary
Ls 3 - lecture #6 - protein separating techniques. In biomolecular experiments, an important concept is the necessity of controls. Important for isolating variables, especially because samples are usually extremely small. Arguably the most useful of these three chromatography techniques because its use of antibodies allows it to isolate very specific aspects of proteins. Positively charged proteins are attracted to negatively charged beads inside a column, and negatively charged proteins are repelled out of the column first. Would expect the first proteins that exit the column to be the most negatively charged, followed by more neutrally charged proteins. To better isolate positive proteins from each other, the charges of the negative beads can be disrupted. A column is filled with beads, and smaller proteins flow through the column slower because they are able to enter pores within beads. Larger proteins exit the column faster because they can"t e(cid:374)te(cid:396) the s(cid:373)all po(cid:396)es in the beads.
