MCD BIO 104AL Lecture Notes - Lecture 8: Enterobacteria Phage T4, Lac Operon, Plasmid
30 Jan 2020
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Complementation: n terminal portion of the protein is encoded by plasmid, c terminus of protein is encoded by an episome, the -gal protein is synthesized in 2 separate pieces and comes together to create a functional enzyme! The entire plasmid doesn"t contain the lacz gene. Can just contain the 5" gene of the lac z gene into the plasmid. When you transform the bacteria with your plasmid, then the plasmid lacz gene will drive the synthesis of the gene. Can come together and create a functioning gene. T4 phage comes from bacteria phage and it is injected into the system. Transfer of amp onto a lysine in an active ligase. Amp is then transferred onto the 5" phosphate of the dna. Preferred outcome: vector plus insert (white colony), but vector could ligate to itself (blue colony) Insert could ligate to itself, can"t really be prevented (no colony), doesn"t have aplicilin gene.
