MCD BIO 165A Lecture Notes - Lecture 1: Myriocin, Colocalization, Phosphatidylserine
11 Feb 2018
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A: the same protein was tagged with gfp and rfp and co-expressed; shows co- localization for the same protein; positive control. D: overlap of proteins with similar sequences, shows higher co-localization of proteins with similar sequences but not as much as the positive control. E: how much do different proteins overlap. A: overlap coefficient: overlap of gfp and rfp, determine if proteins overlap by chance or for functional reason, decoy coefficient: overlap red channel and green channel; channels shouldn"t overlap so it generates a random overlap. B: purple: co-localize for a reason, not randomly, black: random overlap. Figure 6: how external factors influence protein distribution. A and b: phosphatidylserine can be restored to a cho1 mutant by incubating cells in lyso-ps. C: adding back the lipid that was initially disrupted and rescue distribution in some of the proteins, protein distribution is restored for some proteins, e. g. lyso-ps.
