MIMG 101 Lecture Notes - Lecture 11: Phosphoglycerate Kinase, Ethanol Fermentation, Swiss Cheese

• School: UCLA
• Department: Microbiology, Immunology, and Molecular Genetics
• Course: MIMG 101
• Professor: G U N S A L U S& B R A D L E Y

Document Summary

Extraction of energy from organic compounds w/o e- acceptors. Only performed by chemo-organotrophs in anaerobic habitats. Slp (substrate level phosphorylation) to produce energy: transphosphorylate adp -> atp from donor. Pyruvate kinase, phosphoglycerate kinase: atp used to form ion gradients, biosynthesis. Activate -> oxidize -> generate nadh/atp -> recycle nadh -> excrete waste. Use 2 nadh to reduce 2 pyruvate (or carbon intermediate) -> 2 lactate. Nadh is converted back to nad+ in fermentation. Make 2 atp, 2 nadh and pyruvate from glucose. Products: 2 atp, 2 co2, 2 ethanol, nad+ recycled. Yield: 2 atp, 2 nadh, 2 pyruvate. Role in producing lactate for swiss cheese. 3 lactate -> 1 atp, 2 propionate, 1 acetate, 1 co2. Glucose -> 2 ethanol + 2 co2 + 1 atp. 1 atp, nadh, nadph, 2 pyruvate from glucose. 2% of total potential energy from glucose. 93% of energy remains in lactate and is excreted as waste homo-lactic acid bacteria c6 -> 2 c3.