MIMG 101 Lecture Notes - Lecture 19: Rolling Circle Replication, Rna-Dependent Rna Polymerase, T7 Phage

• School: UCLA
• Department: Microbiology, Immunology, and Molecular Genetics
• Course: MIMG 101
• Professor: G U N S A L U S& B R A D L E Y

Document Summary

Use strand as template to make + strand to use as mrna. Make strand + ds intermediate for template. Use strand to make + strand mrna. Use as template to make + strand mrna. Attaches to pili then rna enters but capsid left outside. Attaches own spike proteins to lipoprotein on bacteria. A protein: rf dna synthesis role cleaving and ligating. E protein: host cell lysis by blocking peptidoglycan synthesis. Rolling circle replication: a protein nicks rf + strand -> dna rolls out -> host fills in other strand, a protein cleaves and ligates to get ss dna dsdna bacteriophage t7. Replication: bidirectional, eye and y forms, need to ligate complementary ends together to form concatemer, concatemers, cut so that sticky e(cid:374)d ge(cid:374)e(cid:396)ates 5" ove(cid:396)hang that can be filled. Put t7 promoter upstream of insulin in a plasmid. Put t7 into e coli that is engineered to express t7 pol (transformation)