PHYSCI 121 Lecture Notes - Lecture 7: Laminin, Dystrophin, Myocyte
Document Summary
Experimental approaches: histology, membrane damage assays, molecular assays, physical features, physiology, genetic & chemical manipulations. Interacts with beta-dystroglycan, which interacts with the alpha, which interacts with the laminin. Without laminin, it leads to muscle contraction injuries. Scientist can collect blood and find specific kinase from contraction induced injury. Methods: hemotoxylin & eosin (h&e, van gieson, oil red. Mdx degeneration/regeneration cycles: ages 1 to ~3 weeks, normal muscle morphology, peripheral nucleation. > 3 weeks of age: dystrophic muscle morphology, variation in fiber size, hypertrophic fibers, regeneration = central nucleation, cycle continues leads to fibrosis and infiltration of cells. Nucleus moves to the center of the cell during regeneration. Method to visualize two proteins in muscle: double labeling. Add anti-mouse 2 ab coupled to cy3 (red) Add anti-rabbit 2 ab coupled to fitc (green) Laminin is showing where the extracellular matrix is emhc: regenerating fibers marked by the antibodies.
