MCB 2000 Lecture Notes - Lecture 24: Isocitrate Dehydrogenase, Malate Dehydrogenase, Nadh Dehydrogenase
Document Summary
Tca is 4 redox reactions that transfers electrons. All fuels can enter the tca as acetyl coa of as tca intermediates. Generates (per acetyl group, doubles per glucose molecule: 2 co2, 1 atp (gtp, 3 nadh, 1 fadh2. Tca overview: 8 steps, oaa is key to start cycle and is regenerated, normal ways of cleaving c-c bonds to oxidize won"t work (ex: decarboxylation, have to condense acetate with oaa and carry out beta-cleavage plus oxidation. There are 2 phases: 1: oxidation of acetyl coa to co2, 2: regeneration of oaa. Step 1: citrate synthase performs a condensation reaction between oxaloacetate and acetyl-coa to form citrate. Uses ordered binding: oaa must bind rst in order to reveal the binding site for. Production of oaa is unfavorable, not a lot of oaa is produced. Step 2: aconitase performs an isomerization, citrate isomerize to form isocitrate by aconitase, produces a secondary -oh group.
