MCB 2210 Lecture Notes - Lecture 11: Profilin, Branch Point, Metastasis
27 Oct 2016
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Document Summary
Actin binding proteins: tightly control assembly and disassembly. Treadmilling: when one end is growing and one end of actin is shrinking. Actin nucleating proteins: accelerate the initial kinetics of polymerization, human cells express different actin nucleators that function during distinct processes in different regions of the cell, can help skip lag phase. Formin: form dimers, nucleate long unbranched filaments, fh2 domains, nucleate and remain associated with growing + end of actin, produce long straight single actin filaments. Arp2/3 complex: actin related protein, only nucleators to generate branched filaments, work in conjunction with proteins from the wasp family, wasp family activates allowing branching growth. Localize at membrane: doesn"t start brand new filaments because they bind to side of existing actin filament, branch point from (-) end. Study of actin dynamics aided by the use of drugs: many of the functions of the actin cytoskeleton have been determined by examining the effects of drugs that affect assembly and disassembly, phalloidin.
