MCB 2210 Lecture Notes - Lecture 13: Myh9, Autosomal Recessive Polycystic Kidney Disease, Thrombocytopenia
3 Apr 2016
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Document Summary
Move along actin filaments by coupling the e from atp hydrolysis to conformational changes. 15 classes based on head (motor) domains. Composed of heavy and light chains: heavy chains: Head (motor) domain: contains the actin binding and the. Site of attachment for the regulatory and essential light chains or cam. Tail domains: determine specific properties of each one (what it"ll bind to, whether it"ll be a monomer or dimer, and whether it will polymerize into thick filaments) Differ between myosins: myosin ii: motor protein in muscle that powers contraction. Other isoforms are found in other non-muscle cells. 2 heavy chains an essential and a regulatory light chain. Tails= long alpha-helices that mediate dimerization by forming a coiled coil structure. Also mediate the polymerization of myosin ii into bipolar thick filaments. Actin filament sliding assay: the motor function of myosin can be directly demonstrated by video microscopy of filament sliding assays.
