MCB 2210 Lecture Notes - Lecture 6: Hydrophile, Amphiphile, Trypsin

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sapphiredog102

13 Sep 2016

School

UConn

Department

Molecular and Cell Biology

Course

MCB 2210

Professor

Daggett

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Document Summary

Integral membrane proteins: tightly associated with the lipid bilayer, amino acids interact directly with lipid portion, trans-membrane proteins span the bilayer one or more times while others associate with only one leaflet. Lipid anchored proteins: covalent addition of a lipid to a protein target to the protein to the membrane, fatty acids. Are added to attach proteins to the inner leaflets. Some lipid anchored proteins can cycle between membrane-bound and soluble forms. Peripheral membrane proteins: indirectly attached to the membrane via interactions with other membrane proteins (not lipids) Immunofluorescence or immuno-em: used to immune-localize the protein to the membrane. Purify the membrane and determine which proteins are present. The membranes have a different density than other molecules allowed them to be separated via sucrose gradient centrifugation. Different membranes of the cell have different compositions and can be separated from each other.

Related Questions

Hybridomas are the useful result of a fusion between _______________ and _________________ to obtain hybrid cells that can proliferate and produce _________________ against specific ________________.

	myeloma cells; B lymphocytes; monoclonal antibodies; epitopes
	myeloma cells; T lymphocytes; polyclonal antibodies; antigen
	T lymphocytes; B lymphocytes; monoclonal antibodies; antigen
	T lymphocytes; B lymphocytes; polyclonal antibodies; epitopes

With respect to mammalian cell culture, which of the following statements is true?

	Media used for cell growth contain proteases and a divalent cation chelator to help cells adhere to the surface.
	During the establishment of a cell line, senescence is the last stage of the cell's life.
	Rodent cells cultures undergo spontaneous transformation with similar frequency to human cell cultures.
	Embryonic cell lines do not undergo senescence nor do they have a finite life span.

Epithelial cells have distinct surfaces. Which of these surfaces tends to be involved in secreting proteins out of the cell and into the bloodstream?

	apical
	basal
	All of the answers are correct.
	lateral

Which of the following is NOT true of phospholipid bilayers?

		Phospholipid bilayers separate the cell from the exterior environment as well as from compartments within the cell.
		The interior of phospholipid bilayers is a highly hydrophobic environment.
		Membrane budding requires transient breakages in the bilayer membrane.
		The chemical properties of phospholipids lead to the spontaneous formation of bilayers.

Which of the following is true regarding membrane proteins?

		All peripheral membrane proteins contact the lipid bilayer by covalent attachment to the hydrophilic head groups on membrane lipids.
		Beta strands can assemble into a membrane-spanning barrel structure.
		Lipid-anchored membrane proteins always face the cell exterior.
		A protein with six hydrophobic helices is most likely a lipid-anchored membrane protein.

Which of the following statements is NOT true?

		The three types of lipid anchors responsible for tethering proteins to the bilayer are acylation, prenylation, and ubiquitinylation.
		Movement of lipids from the cytoplasmic to the exoplasmic leaflet of the membrane bilayer requires specialized transport proteins called flippases.
		Glycoproteins are the basis for blood type compatibility between donor and receiver.
		Glycoproteins are always oriented so that the carbohydrate moieties are exoplasmic.

MCB 2210 Lecture Notes - Lecture 6: Hydrophile, Amphiphile, Trypsin

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