MCB 2400 Lecture Notes - Lecture 3: Taq Polymerase, Dna Replication, Telomerase
5 Nov 2018
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Document Summary
Dna sequencing: determine the identity/order of bases in dna molecule. Ingredients in sequencing reaction: template dna (recombinant dna, sequencing primer two primers (only one direction, 2 types of nucleotides, dntps, ddntps, buffer, taq dna polymerase. Notice how the ddntps do not have an oh group on the 3" carbon, this is is what causes termination due to lack of hydroxyl group (binding site) Sequencing results only make sense when one strand is used as a template at a time. Challenges of dna replication specific to eukaryotes: dna is packaged in nucleosomes. Nucleosomes are displaced by the replication fork and are rapidly reassembled onto dna new and old histones are randomly assembled onto the 2 daughter strands. Circular chromosome of bacteria; linear chromosome of humans. Telomerase activity is needed for replication of telomeres. Without telomerase, the chromosome is shortened each time the cell divides causing the dna to be eroded and the chromosome is damaged.
