EEMB 129 Lecture Notes - Lecture 22: Genome Size, Plasmid, Intron
15 Nov 2017
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Strategy: sequence smaller pieces with overlapping parts and then piece them back together into larger contigs Most we can sequence at 1 time: 1,000 bp. Polymorphism the occurrence of two or more alleles at one locus, each with appreciable frequency. Genomes are diploid (mom"s dna differs from dad"s) Hard to tell if it is error or polymorphism. No longer a unique sequence so don"t know where the sequence goes. Some sequences are repeated all over the genome. Classic sequencing works by extension from a primer followed by gel electrophoresis. Genome size is poorly correlated with organismal complexity (more complex organism doesn"t mean larger genome size) Long insert vectors can link smaller scaffolds into larger scaffolds. Large scaffolds can be linked with genetic mapping. Generate nested array of fragments; each with a fluorescent label corresponding to the terminating 3" base (no more bp can be added)
