BICD 100 Lecture Notes - Lecture 6: Ribosomal Rna, Start Codon, Transfer Rna

18 views4 pages

marooncrocodile139

19 Dec 2016

School

UCSD

Department

Biol/Genetics,Cellular & Develop

Course

BICD 100

Professor

Keefe Reuther

For unlimited access to Class Notes, a Class+ subscription is required.

Document Summary

Information transfer in the cell (central dogma: dna replication, transcription from dna to rna, translation from mrna to polypeptide. Molecular components of transcription: rna synthesis is catalyzed by rna polymerase, which pries the dna strands apart and hooks together the rna nucleotides. Rna polymerase not a single enzyme, many rna polymerases that all do the same basic thing, used to code different types of genes that give us different types of rna. Different from dna polymerase bc dna polymerase does not split apart strands (cid:894)does(cid:374)"t (cid:271)(cid:396)eak h (cid:271)o(cid:374)ds --> that"s dna heli(cid:272)ase"s jo(cid:271)(cid:895) Does(cid:374)"t i(cid:374)(cid:272)lude p(cid:396)o(cid:373)ote(cid:396), (cid:449)hi(cid:272)h is (cid:396)e(cid:272)og(cid:374)itio(cid:374) site that establishes beginning of transcription unit and which strand is the template strand transcription unit is smaller than overall gene, within transcription unit: open reading frame distance between start and stop codon. Need rna segments before and after transcription unit to regulate process of translation.

Related Questions

Biochem:

1. Indicate whether the following describes DNA polymerase, RNApolymerase, both, or none:

a. Binds to single stranded DNA

b. Uses an RNA primer

c. Has proof-reading ability

d. Reads from 3' to 5'

e. Phosphodiester bonds are formed using rNTPs

f. Requires a DNA primer

g. Multiple types are present in eukaryotes

h. Mutiple types are present in prokaryotes

i. Is synthesized bi-directionally

j. Requires there to be a terminal sequence on DNA

k. Is part of a larger complex made up of several proteins

2. Post-translational damage causes the loss of the 2' hydoxylgroup on several adenosine nucleotides within the intron sequenceof a primary RNA transcript. What could possible happen to theresulting mature RNA sequence and consequent protein?

3. Where on the below strand of RNA would you most likely findthe following:

a. Start of transcription

b. Start of translation

c. TATA box

d. Enhancers

e. Initiator

f. Activators

g. Repressors

4. How would the following mutation in the template strand ofDNA affect the translated protein? (x=any base, / = exon/intronjunction)

5-XXXXC/CTGAAGAGGAAGXXXXGTTAGXXXXACTCAC/CTCGC-3 to5-CCTGAAGAGGAAXXXXXXXXGTTAGXXXXACTCACCTCGC-3

5. When will there be a 5'-5' linkage in RNA? When is this bondformed? Which base is commonly involved in this process? What wouldhappen if there was an inability in this enzyme to make the 5'-5'likage?

6. Consider the following strand of mRNA. What protein would beproduced if this strand was translated?

5'- CGUAUGCCCUUUACUAGUAUGUGAUACUAGUAAACC-3'

7. What would be the new protein if the following mutations weremade to the strand in 6? Label/classify each type of mutation.

a. 5'-CGUAUGCCCUUUAACUAGUAUGUGAUACUAGUUAACC-3'

b. 5'-CGUAUGCCCUUCACUAGUAUGUGAUACUAGUUAACC-3'

c. 5'- CGUAUGCCCUUUACUAGUAUUGAUACUAGUUAACC-3'

d. 5'- CGUAUGCCCUUGACUAGUAUGUGAUACUAGUAAACC-3'

e. 5'-CGUAUGCCCUUCACUAGUAUGAGUAACUAGUAACC-3'

f. 5'-CGUAUGCCCUAAACUAGUAUGUGAUACUAGUAAACC-3'

8. What are examples of two different types of point mutationsin sequences coding for hemoglobin production?

pearbeaver961

BICD 100 Lecture Notes - Lecture 6: Ribosomal Rna, Start Codon, Transfer Rna

Document Summary

Get access

Related Documents

BICD 100 Study Guide - Midterm Guide: Gene Expression, Start Codon, Transfer Rna

BICD 100 Lecture Notes - Lecture 6: Transfer Rna, Central Dogma Of Molecular Biology, Information Transfer

BICD 100 Study Guide - Midterm Guide: Thermal Cycler, Genbank, Gene Expression

Related Questions