BICD 100 Lecture Notes - Lecture 18: Hayflick Limit, Telomerase, Wild Type
1 Apr 2019
School
Department
Course
Professor
Document Summary
Focus on the biology of the aging process. Human cells have a clock that counts cell divisions. Cells placed in media and allowed to divide could only divide for so long, even if frozen and allowed to start again. Dna polymerases can only synthesize dna in one direction 5"-3" The "lagging strand" synthesized in many short pieces. Dna polymerases need an rna primer to get started. The ends of chromosomes have an unusual feature. Ciliates have a part of their lifecycle where they make millions of chromosome ends. They have a distinctive feature of a dna sequence on the ends of chromosomes. A telomere dedicated dna polymerase, which adds telomeric repeats onto chromosome ends. Telomerase is hard to detect in human cells. Pcr used to amplify the products of telomerase. 90 out of 101 biopsies of cancer: telomerase positive week 10 page 1. 90 out of 101 biopsies of cancer: telomerase positive. 0 out of 50 normal tissues have telomerase.
