BICD 110 Lecture Notes - Lecture 18: Cyclin-Dependent Kinase, Tunel Assay, Epidermal Growth Factor Receptor
Document Summary
Lecture 9. 1 cell cycle #2 read 1151-1153 on class webpage. Cyclin/cdk complexes (egf, pdgf: regulatory cyclin plus (carpenter) + catalytic cyclin dependent kinase cdk (hammer) Growth factor receptors: growth factors bind a cell surface receptor (egf/pdgf tyrosine kinase, growth factor signal transduction cascade initiates the cell. Cdk/cyc turns on dna replication (s phase) g2 --- (cdk1/cyclin b) m . P53 (tsg): guardian of the genome: add to top signaling pathway dna damage p53 notices dna damage and becomes more stable p53 is tf that turns on cip gene by binding upstream . Mutations in these genes cause cancer: proto ongogenes involved in normal signaling pathways/cellular genes that control cell growth, mutate to oncogenes, discovery of first cancer causing gene (src by michael bishop and harold. Varmus) rous sarcoma virus caused cancer in chickens. G2 (with cdk/cyclin b aka mitotic kinase) m 2 cells!
