BIMM 100 Lecture Notes - Lecture 9: Transposase, Dna Profiling, Transfer Rna

• School: UCSD
• Department: Biology/Molec Biol, Microbiol
• Course: BIMM 100
• Professor: Amy Pasquinelli

Document Summary

If the (cid:272)hild (cid:271)a(cid:374)d does(cid:374)"t (cid:373)at(cid:272)h with a (cid:373)other, look for father"s (cid:271)a(cid:374)d that (cid:373)at(cid:272)hes up. If (cid:272)hild"s (cid:271)a(cid:374)d is (cid:374)ot fro(cid:373) (cid:373)other, (cid:373)ust (cid:271)e from father. Reverse transcription: discovered in retrovirus: when virus infects animal, genetic material injected is single stranded rna. Virus also injects proteins that allows dna to be created from rna (rna dependent dna polymerase activity: need primer from trna in translation machinery from the host cell to synthesize. Dna strand: degrade rna strand to leave single-stranded dna using rnase, copy dna into 2 double strands: dna-dependent dna polymerase activity, reverse transcriptase: viral enzyme that functions as 3 enzymes (rna dependent. Dna polymerase, dna dependent dna polymerase, and rnase: virus integrates double stranded dna into host genome using integrase (viral enzyme). Eukaryotic cell reverse transcription: mobile elements: discovered that some dna can move within and between chromosomes. Then synthesize 2nd dna strand and insert this strand into another location.