BILD 1 Lecture Notes - Lecture 5: Scanning Electron Microscope, Microscopy, Cytosol
Document Summary
Robert hooke: designed light microscope to study cells (protists, blood cells, tree tissue) Anton van leeuwenhoek: used microscopes to study microbes, any living thing. Electron microscopy: provides highest resolution: specimen must be dead; looking at them under a vacuum, sem (scanning electron microscopy): surface features of specimen, tem (transmission electron microscopy): thinly sliced specimen bombarded with e- Light microscopy: used to study processes in living cells: can track how things move around/how cells divide, can label individual cells with fluorescent markers, can use hard/software to get better resolution (confocal microscopy/deconvolution) 3 domains of cells: eukarya, archaea, and bacteria: bacteria and archaea: prokaryotic cells, eukarya (protists, fungi, animals, plants): eukaryotic cells. Cells have maximum size limit: metabolic requirements set upper limits on size. As cell increases in size, volume grow proportionately more than its surface area, so not enough energy created to sustain cell if too large. Encompassed by nuclear envelope, consisting of inner and outer membrane.
