BIMM 110 Lecture Notes - Lecture 5: Wild Type, Brca1, P53
25 Feb 2017
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Barriers: viruses immune responses, renewal of cells, must continually reintroduce. Introduced in thousands of cells rare occasion: start will cell where we know homologous recombination has occurred grow the whole mouse from that cell, make this rare event happen reliably in most living cells . Problem: some genes are so important in many tissues and processes, that knocking them out results in no viable animals. Solution: conditional ko : mutation happens only in certain tissue or at a certain time. Cre recombinase, recognizes loxp sequences (originally found in p1 bacteriophage) or flp recombinase, recognizes frt sequences (from yeast: allows us to control where and when a ko or ki can occur. General principle: using the surrounding exon you want to delete with loxp sites. Express cre recombinase only in tissue where you want to generate the ko. Cre will use loxp sites to delete dna sequences between them.
