BIMM 121 Lecture Notes - Lecture 3: Antonie Van Leeuwenhoek, Oil Immersion, Coccobacillus
26 Oct 2018
School
Department
Course
Professor
Document Summary
The space is filled with air, causing refraction to occur. Therefore, not enough light hits objective lens and a clear image cannot be created: mineral oil (immersion oil) prevents light rays from bending away from the objective lens. Yeast cells: they are eukaryotes, saccharomyces cerevisiae can undergo mitosis through budding, small daughter cell buds from mother cell. Staining cells to visualize under microscope: simple stain, allows us to give color to cells, fix cells. Peptidoglycan usually attached to s proteins: gram negative (-): stain pink/red, thin peptidoglycan. Contains outer membrane: lipopolysaccharides (lps), lipoproteins, and phospholipids: lps: lipid a, the core polysaccharide, o side chain, difference in color reflects difference in cell wall composition, procedure, bacteria stay on slide through heat fixation. Wait until slide is completely dry before heat fixation, or else the bacteria will boil: crystal violet: flood heat-fixed smear with crystal violet. Gram + cells have thick layer of peptidoglycan, so.
